Method of amplifying the signal of target nucleic acid sequence analyte
US6054274A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Nov 12, 1997 |
| Grant date | Apr 25, 2000 |
| Priority date | — |
| Expiry date | Nov 12, 2017 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/682
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method is disclosed of amplifying the signal of target nucleic acid sequence analyte using a rolling circle replication mechanism and a bidirectional primer. The repeating signal amplification sequence units contain tags which are directly or indirectly detectable. In addition, methods of capturing the tagged complementary nucleic acid sequence of the target nucleic acid sequence onto an array surface and detecting the captured target nucleic acid sequences are disclosed. Kits are also disclosed for enhancing detection of target nucleic acid sequences using a mechanism of rolling circle replication and a bidirectional primer to attach to the complementary nucleic acid sequence of the target nucleic acid sequence a large number of detectable tags.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.