Cyclosporin immunoassay

US6054303A · kind A · utility

Assignee

• Dade Behring Marburg GmbH · DE

Inventors

• Dariush Davalian · San Jose, US
• Maureen Beresini · Moss Beach, US
• Svetlana Alexander · Sunnyvale, US
• Mae W. Hu · Los Altos, US
• Edwin F. Ullman · Atherton, US

Key dates

• Filing date: Mar 10, 1995
• Grant date: Apr 25, 2000
• Expiry date: Mar 10, 2015

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S530/807
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A method of measuring the amount of cyclosporin in a sample suspected of containing cyclosporin is disclosed. A method of inactivating interfering cross-reactive material in an assay for measuring the amount of cyclosporin in a sample suspected of containing cyclosporin is also disclosed. Compositions wherein cyclosporin is conjugated to an immunogenic carrier or a label, optionally through a linking group, at an alanine nitrogen atom of the cyclic backbone of cyclosporin are also disclosed. Compositions wherein atiocyclosporin is conjugated, optionally through a linking group, to an immunogenic carrier or a label are also disclosed. Where cyclosporin is conjugated to an immunogenic carrier, the conjugates may be used as immunogens for the preparation of antibodies which are capable of recognizing cyclosporin. Where atiocyclosporin is conjugated to an immunogenic carrier, the conjugates may be used as immunogens for the preparation of antibodies which are capable of recognizing interfering cross-reactive material but substantially incapable of recognizing cyclosporin or cyclosporin-label conjugates. Where cyclosporin is conjugated to a label, the conjugates may be used as part of a…