Thermostable ligase mediated DNA amplification system for the detection of genetic diseases
US6054564A · kind A · utility
111Cited by
4References
19Claims
0Family size
Assignees
Inventors
Key dates
| Filing date | Oct 7, 1997 |
| Grant date | Apr 25, 2000 |
| Priority date | — |
| Expiry date | Oct 7, 2017 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6858
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to the cloning of the gene of a thermophilic DNA ligase, from Thermus aquaticus strain HB8, and the use of this ligase for the detection of specific sequences of nucleotides in a variety of nucleic acid samples, and more particularly in those samples containing a DNA sequence characterized by a difference in the nucleic acid sequence from a standard sequence including single nucleic acid base pair changes, deletions, insertions or translocations.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.