Chondroitin lyase enzymes
US6054569A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Sep 17, 1997 |
| Grant date | Apr 25, 2000 |
| Priority date | — |
| Expiry date | Sep 17, 2017 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N9/88
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention describes a method for the production of two highly purified enzymes capable of degrading chondroitin sulfate polysaccharides. A multi-step purification method incorporating cell disruption, cation exchange chromatography, affinity chromatography, hydroxylapatite chromatography, high resolution ion exchange chromatography and size exclusion is outlined. A 77,000.+-.5,000 Dalton protein capable of degrading chondroitin sulfates A and C and a 55,000.+-.2,300 Dalton protein capable of degrading dermatan sulfate were isolated. The genes encoding these enzymes, chondroitinase AC and chondroitinase B, respectively, have been cloned and methods for their use are described.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.