Method of making monoclonal antibodies using polymorphic transgenic animals
US6054632A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Nov 15, 1996 |
| Grant date | Apr 25, 2000 |
| Priority date | — |
| Expiry date | Nov 15, 2016 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA01K2267/01
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention relates to a method for making monoclonal antibodies having pre-defined specificity for an epitope characteristic of or unique to a single form of a polymorphic protein. The method includes constructing a first transgenic animal to express a first form of a polymorphic protein encoded by a first allele of a gene encoding the protein; constructing a second transgenic animal to express a second form of the polymorphic protein encoded by a second allele of the gene encoding the protein; and immunizing the first transgenic animal with cells from the second transgenic animal expressing the second form of the polymorphic protein to induce an immune response in the first transgenic animal yielding an antibody specific for an epitope peculiar to the second form of the polymorphic protein. The invention further includes hybridoma cells secreting a monoclonal antibody specific for the second form of the protein. The invention is particularly advantageous in the context of making monoclonal antibodies and derivative reagents specifically identifying polymorphic blood group proteins, such as the Duffy gp-Fy protein.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.