Methods for identifying biological macromolecule interactions with compounds, particularly in complex mixtures
US6054711A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Nov 12, 1997 |
| Grant date | Apr 25, 2000 |
| Priority date | — |
| Expiry date | Nov 12, 2017 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2021/3595
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Methods for determining whether a biological macromolecule binds to a compound are disclosed. The methods include: a) obtaining a Fourier-Transform Infrared spectrum of a solution which contains a biological macromolecule and obtaining a Fourier-Transform Infrared spectrum of a solution which includes a compound; b) obtaining a Fourier-Transform Infrared spectrum of a solution which contains the biological macromolecule and compound of step a); c) subtracting the Fourier-Transform Infrared spectrum of the biological macromolecule of step a) and the Fourier-Transform Infrared spectrum of the compound of step a) from the Fourier-Transform spectrum of step b) to determine a subtracted index; and d) determining that the compound of step a) binds to the biological macromolecule of step a) when the subtracted index of step c) is not equal to zero.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.