Apparatus for preparing skin cell culture
US6057148A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Feb 9, 1998 |
| Grant date | May 2, 2000 |
| Priority date | — |
| Expiry date | Feb 9, 2018 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2533/54
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A process for preparing a culture skin which comprises the following steps: PA0 a) a step in which a culture skin matrix comprising a collagen sponge, a collagen sheet or a collagen gel is prepared in a means having a projection to provide said matrix with a penetrating pore, PA0 b) a step in which a skin-derived cell is seeded and cultured on said matrix, and PA0 c) a step in which the culture skin is provided with a penetrating pore by the means having the projection before said culture skin covers a skin defect, if necessary, the steps a) and b) are simultaneously carried out; a culture skin matrix and a culture skin obtained thereby. According to the present invention, a culture skin can be prepared wherein sufficient nutrition supply to epidermal cells and/or fibroblasts cultured and proliferated and discharge of exudate excessively pooled in the wound can be smoothly performed, while contraction is inhibited, by producing a penetrating pore in the culture skin before covering the wound with the culture skin with a means which requires no pretreatment in order to prevent the flow and loss of cells which are caused in seeding cells on a collagen matrix.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.