Reagent and method for isolation and detection of selected nucleic acid sequences
US6060246A · kind A · utility
92Cited by
6References
18Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Nov 13, 1997 |
| Grant date | May 9, 2000 |
| Priority date | — |
| Expiry date | Nov 13, 2017 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/1006
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention relates to compositions and methods for rapidly detecting or isolating a target nucleic acid sequence in a polynucleotide-containing sample. The sample is exposed to a rapid pairing reagent, which contains a rapid capture component, effective to rapidly and non-selectively bind polynucleotides, and a target specific probe, effective to selectively bind the target nucleic acid sequence. Selectively disrupting the binding between the capture component and polynucleotides leaves only target sequence bound to the rapid pairing reagent.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.