Type IV collagen high molecular form and production and diagnostic use thereof
US6060255A · kind A · utility
Assignees
Inventors
Key dates
| Filing date | Oct 1, 1998 |
| Grant date | May 9, 2000 |
| Priority date | — |
| Expiry date | Oct 1, 2018 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S530/846
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A type IV collagen high molecular form having a higher molecular weight than the 7S domain of type IV collagen and including the 7S domain in its structure, is obtained from the supernatant being recovered from a collagen solution digested by pepsin in the following steps; PA1 1) salt precipitating with sodium chloride at a concentration no higher than 1.2 M, PA1 2) dissolving the precipitates, PA1 3) salt precipitating with sodium chloride at a concentration no higher than previous concentration. By reacting a sample with an antibody which reacts with this form, the type IV collagen high molecular form in the sample can be measured to allow diagnosis of the degree of liver fibrosis in patients with liver diseases.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.