Process for the production of hetero-bispecific antibodies

US6060285A · kind A · utility

Assignee

• Roche Diagnostics GMBH · DE

Inventors

• Helmut Lenz · Tutzing, DE
• Ulrich Weidle · Hersbruck, DE

Key dates

• Filing date: Mar 22, 1990
• Grant date: May 9, 2000
• Expiry date: Mar 22, 2010

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S435/972
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

For the production of hetero-bispecific monoclonal antibodies at least the genes for the light chain and for the variable part of the heavy chain are isolated from a hybridoma cell line which secretes an antibody with a desired specificity and they are inserted into a eukaryotic plasmid vector which contains a marker capable of selection together with a strong promoter, this expression vector is transfected into a hybridoma cell line which secretes antibodies with a second desired specificity, the cell line is cultured, the antibodies are obtained and the bispecific antibody is isolated.