Apparatus and method for sterilizing, seeding, culturing, storing, shipping and testing replacement cartilage tissue constructs
US6060306A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Aug 14, 1997 |
| Grant date | May 9, 2000 |
| Priority date | — |
| Expiry date | Aug 14, 2017 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA61L2430/06
- WIPO fieldMedical technology
- WIPO sectorInstruments
Abstract
An apparatus and method for sterilizing, seeding, culturing, storing, shipping, and testing cartilage constructs is disclosed. The apparatus generally includes a treatment chamber and a means for varying the fluid flow and pressure within the treatment chamber during seeding and culturing. The application of pressure to the cartilage construct improves both the uniformity of seeding throughout the construct and facilitates the flow of nutrients to and removal of waste products from cells embedded in the construct. In this manner, a tissue-engineered cartilage construct with cells and their fibers oriented in a manner which is more likely to possess long term dimensional stability and the patency of cartilage with normal physiologic function may be formed.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.