Polynucleotide separations on polymeric separation media
US6066258A · kind A · utility
23Cited by
17References
9Claims
0Family size
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Key dates
| Filing date | Oct 30, 1998 |
| Grant date | May 23, 2000 |
| Priority date | — |
| Expiry date | Oct 30, 2018 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/101
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
Non-polar polymeric separation media, such as beads or monoliths, are suitable for chromatographic separation of mixtures of polynucleotides when the surfaces of the media are unsubstituted or substituted with a hydrocarbon group having from one to 1,000,000 carbons and when the surfaces are substantially free from mutivalent cation contamination. The polymeric media provide efficient separation of polynucleotides using Matched Ion Polynucleotide Chromatography. Methods for maintaining and storing the polymeric media include treatment with multivalent cation binding agents.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.