Modified green fluorescent proteins

US6066476A · kind A · utility

Assignee

• The Regents of the University of California · US

Inventors

• Roger Y. Tsien · Village of La Jolla, US
• Roger Heim · Del Mar, US

Key dates

• Filing date: Nov 20, 1996
• Grant date: May 23, 2000
• Expiry date: Nov 20, 2016

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S530/855
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Modifications in the sequence of Aequorea wild-type GFP provide products having markedly different excitation and emission spectra from corresponding products from wild-type GFP. In one class of modifications, the product derived from the modified GFP exhibits an alteration in the ratio of two main excitation peaks observed with the product derived from wild-type GFP. In another class, the product derived from the modified GFP fluoresces at a shorter wavelength than the corresponding product from wild-type GFP. In yet another class of modifications, the product derived from the modified GFP exhibits only a single excitation peak and enhanced emission relative to the product derived from wild-type GFP.