Method for high specific bioproductivity of .alpha.,.omega.-alkanedicarboxylic acids
US6066480A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Sep 21, 1998 |
| Grant date | May 23, 2000 |
| Priority date | — |
| Expiry date | Sep 21, 2018 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12P7/44
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
This invention provides a low-cost method of producing .alpha.,.omega.-alkanedicarboxylic acids. Particular bioconversion conditions result in highly efficient conversion of fatty acid, fatty acid ester, or alkane substrates to diacids. Candida tropicalis AR40 or similar yeast strains are grown in a medium containing a carbon source and a nitrogen source at a temperature of 31.degree. C. to 38.degree. C., while additional carbon source is continuously added, until maximum cell growth is attained. Within 0-3 hours of this point, substrate is added to the culture to initiate conversion. An .alpha.,.omega.-alkanedicarboxylic acid made according to this method is also provided.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.