Apparatus for culturing mammalian respiratory epithelial
US6066496A · kind A · utility
Inventor
Key dates
| Filing date | Mar 9, 1998 |
| Grant date | May 23, 2000 |
| Priority date | — |
| Expiry date | Mar 9, 2018 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2501/81
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method of culturing non-transformed mammalian respiratory epithelial cells in vitro. The method includes the steps of harvesting the epithelial cells from the respiratory tract of a mammalian donor; suspending the epithelial cells in a fluid culture medium to form a cell suspension; introducing the cell suspension into a first reservoir in communication with an adherence substrate; introducing additional fluid culture medium into a second reservoir separated from the first reservoir by a fluid permeable baffle; incubating the cells under conditions suitable for promoting epithelial cell growth for a period of at least 72 hours; and changing the fluid culture medium within the second reservoir during the incubation period at intervals of approximately 24-72 hours without disturbing the epithelial cells within the first reservoir. Preferably, the epithelial cells are harvested from the nasal mucosa of a human donor using a minimally-invasive brushing technique. A novel culture dish suitable for carrying out the method is also described. The culture dish includes first and second reservoirs for holding fluid culture medium; a fluid permeable first baffle separating the first and sec…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.