Simplified sequential chemiluminescent detection
US6068979A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Apr 22, 1998 |
| Grant date | May 30, 2000 |
| Priority date | — |
| Expiry date | Apr 22, 2018 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/973
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method for sequential chemiluminescent detection of two differently labeled analytes on a single blot is described. In the method, a uniquely labeled DNA is detected with a horseradish peroxidase (HRP) substrate followed by the detection of another uniquely labeled DNA with a second different enzyme substrate which also inhibits the chemiluminescence generated by HRP. The sequential detection method described herein eliminates the need to strip and reprobe Southern, Northern and Western blots. Potential applications of this method include forensic DNA fingerprinting where more than one probe is used for probing a Southern blot, multiplex DNA sequencing of more than one template, detection of gene rearrangements, mutations and gene linkage.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.