Detection of microbial metabolites with a 3-indoxyl-myo-inositol-1-phosphate compound
US6068988A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Dec 10, 1997 |
| Grant date | May 30, 2000 |
| Priority date | — |
| Expiry date | Dec 10, 2017 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/883
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method of detecting a phosphatidylinositol-specific phospholipase C enzyme by means of a substrate which is cleaved by said enzyme and yields a dye when the chromophoric portion of the substrate is dimerized and oxidized; the invention teaches using in such method, as a novel substrate, a 3-indoxyl-myo-inositol-1-phosphate compound of formula (I) ##STR1## wherein R is selected from the group consisting of hydrogen and C.sub.1-4 alkyl, while R.sub.1, R.sub.2, R.sub.3, and R.sub.4 are radicals selected from the group consisting of hydrogen, halogen, cyano, nitro, carboxy, amino, alkyl-substituted amino and sulphonyl; or of a salt of said formula I compound. The invention provides for a safe and sensitive method of detection of potentially pathogenic bacterial activity of such microbes as Bacillus cereus, B. Thuringiensis, Staphy lococcus aureus and various Listeria strains in potentially infected materials including physiological samples or consumable goods such as foods and beverages.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.