Method for carbohydrate engineering of glycoproteins
US6069235A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Feb 23, 1994 |
| Grant date | May 30, 2000 |
| Priority date | — |
| Expiry date | Feb 23, 2014 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/924
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method is disclosed for modifying the carbohydrate moiety on glycoproteins to facilitate the structural and functional analysis of said glycoproteins such as by NMR spectroscopic analysis and crystallography which comprises treating glycoprotein-secreting mammalian cells having low endomannosidase activity under cell culture maintenance conditions with a glucosidase I inhibitor, and after secretion and purification, subsequent treatment of the active glycoprotein with endoglycosidase H to thereby provide a glycoprotein with a single GlcNAc residue at each glycosylation sequon. The preferred mammalian cells are CHO cells and the preferred glucosidase I inhibitor is N-butyl deoxynojirimycin.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.