Channel-less separation of bioparticles on a bioelectronic chip by dielectrophoresis
US6071394A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jan 30, 1998 |
| Grant date | Jun 6, 2000 |
| Priority date | — |
| Expiry date | Jan 30, 2018 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB01L2400/0644
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
The present invention comprises devices and methods for performing channel-less separation of cell particles by dielectrophoresis, DC high voltage-pulsed electronic lysis of separated cells, separation of desired components from crude mixtures such as cell lysates, and/or enzymatic reaction of such lysates, all of which can be conducted on a single bioelectronic chip. A preferred embodiment of the present invention comprises a cartridge (10) including a microfabricated silicon chip (12) on a printed circuit board (14) and a flow cell (16) mounted to the chip (12) to form a flow chamber. The cartridge (10) also includes output pins (22) for electronically connecting the cartridge (10) to an electronic controller. The chip (12) includes a plurality of circular microelectrodes (24) which are preferably coated with a protective permeation layer which prevents direct contact between any electrode and a sample introduced into the flow chamber. The permeation layer also helps to reduce cell adhesion at field minima, and enables immobilization of specific antibodies for specific cell capture. Specific cells from various cell mixtures were separated, lysed, and enzymatically digested on the…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.