Process and culture medium for the production of cells infected by a multiple sclerosis-associated virus
US6071736A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Nov 20, 1996 |
| Grant date | Jun 6, 2000 |
| Priority date | — |
| Expiry date | Nov 20, 2016 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2469/20
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
In a process for the in vitro production of a culture or cell line infected by a viral strain associated with multiple sclerosis (MS), a body sample is taken from an individual suffering from MS. The sample is cultivated in a culture medium that promotes the growth of infected cells to obtain a culture of primary infected cells. A sample of the culture of primary cells or a subculture of the latter is cultivated in series, by successive passages in the culture medium to obtain the culture or cell line infected by a virus associated with MS. The culture medium also contains a beta-interferon antibody or an antibody that is directed against an antigenically close molecule, the antibody playing an inhibiting role in viral expression and allowing long-lasting expression and propagation of the viral strain in the culture or cell line.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.