Microfluidic method for nucleic acid purification and processing

US6074827A · kind A · utility

Assignee

• Aclara Biosciences, Inc. · US

Inventors

• Robert J. Nelson · Sanford, US
• Herbert H. Hooper · Belmont, US
• Alan K. Hauser · Palo Alto, US
• Sharat Singh · San Jose, US
• Stephen J. Williams · Scottsdale, US
• Alexander Sassi · Berkeley, US

Key dates

• Filing date: Feb 5, 1998
• Grant date: Jun 13, 2000
• Expiry date: Feb 5, 2018

Classification

• Technology area (CPC B): Performing Operations; Transporting
• CPC primary: B01L2400/0421
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Integrated microfluidic devices comprising at least an enrichment channel and a main electrophoretic flowpath are provided. In the subject integrated devices, the enrichment channel and the main electrophoretic flowpath are positioned so that waste fluid flows away from said main electrophoretic flowpath through a discharge outlet. The subject devices find use in a variety of electrophoretic applications, including clinical assays, high throughput screening for genomics and pharmaceutical applications, point-or-care in vitro diagnostics, molecular genetic analysis and nucleic acid diagnostics, cell separations, and bioresearch generally.