Rapid assay for infection in neonates
US6077665A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Apr 8, 1997 |
| Grant date | Jun 20, 2000 |
| Priority date | — |
| Expiry date | Apr 8, 2017 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/56988
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A rapid assay for infection in immunodeficient patients such as neonates or immunocompromised patients (e.g. HIV or transplant patients) allows diagnosis at initial evaluation, such that antibiotic treatment and confinement to an intensive care unit can be avoided for uninfected patients. The assay can be used for detecting bacterial, viral, or fungal colonization of the blood stream, cerebrospinal fluid (CSF), or urinary tract. The method is particularly useful for sepsis diagnosis. Polymorphonuclear leukocyte (PMN, neutrophil) CD11b (Mac-1, CR3) levels are measured by flow cytometry or laser scanning microscopy in low volume (0.1 ml) whole blood samples. A dual-laser FACS identifies neutrophils by FITC-conjugated anti-CD15 fluorescent antibodies, and identifies surface neutrophil CD11b marked with PE-conjugated anti-CD11b antibodies. Spontaneous upregulation of CD11b is prevented by handling samples at 4.degree. C. or adding a stabilizing compound such as anti-CD14 antibody or adenosine to the samples. A kit comprises calibration markers such as CD11b-coated microspheres for calibrating the device used for analysis according to diagnosis threshold(s).
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.