Highly sensitive multimeric nucleic acid probes
US6077668A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Aug 13, 1997 |
| Grant date | Jun 20, 2000 |
| Priority date | — |
| Expiry date | Aug 13, 2017 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2310/53
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
The present invention provides detectably labeled RNA and DNA oligonucleotide multimers useful as diagnostic probes in medical, biological and chemical applications. A method for synthesizing DNA and RNA oligonucleotides, oligonucleotide multimers, and analogs, preferably those that are detectably labeled, is also provided. Oligonucleotide synthesis is performed by combining a circular single-stranded oligonucleotide template with an effective polymerase and at least two types of nucleotide triphosphate, without the addition of auxiliary proteins, to yield an oligonucleotide multimer comprising multiple copies of a repeated oligonucleotide sequence.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.