Method of producing oligonucleotide arrays with features of high purity
US6077674A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Oct 27, 1999 |
| Grant date | Jun 20, 2000 |
| Priority date | — |
| Expiry date | Oct 27, 2019 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC40B40/06
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
A method of making full-length oligonucleotide arrays provides for the purification of pre-synthesized full-length oligonucleotides from shorter length oligonucleotides and other impurities at the same time the oligonucleotides are deposited on the array. A synthesized mixture that includes desired full-length oligonucleotides and some capped shorter length or "failed" oligonucleotide sequences, is reacted with a linking agent to add a linking group on to the free-end of the full-length oligonucleotides but not the shorter-length oligonucleotides. The resulting mixture is deposited on an array without first separately purifying the mixture to remove the unwanted shorter-length oligonucleotides. After deposition, unbound material, including the shorter length oligonucleotide sequences and other impurities, is removed.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.