Glycosylhydrazines preparation immobilization and reactions of glycoprotein analysis and O-glycan removal

US6077951A · kind A · utility

Inventors

• John William Redmond · Parramatta, AU
• Nicolle Hannah Packer · Chatswood, AU
• Andrew Arthur Gooley · Parramatta, AU
• Keith Leslie Williams · Seattle, US
• Michael Batley · Hornsby, AU
• Warren Kett · Chatswood, AU
• Anthony Pisano · Tuncurry, AU
• Helen Joan Tweeddale · Hornsby, AU
• Catherine Anne Cooper · Hornsby, AU

Key dates

• Filing date: Nov 3, 1998
• Grant date: Jun 20, 2000
• Expiry date: Nov 3, 2018

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C09B29/366
• WIPO field: Organic fine chemistry
• WIPO sector: Chemistry

Abstract

The present invention consists in methods of preparing derivatives either isolation or from glycopeptides or glycoproteins. The methods comprise producing sugar hydrazones, sugar pyrazoles, glycosylpyrazones, azoglycan dyes and hydrazoglycan dyes. The present invention also relates to the removal of O-glycans from glycopeptides or glycoproteins, immobilising reducing sugars onto solid supports and methods to obtain sugars from glycopeptide or glycoprotein comprising subjecting the glycopeptide or glycoprotein to solid-phase Edman degradation followed by separating and characterizing the sugars.