Internalisation of DNA, using conjugates of poly-l-lysine and an integrin receptor ligand
US6083741A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | May 16, 1997 |
| Grant date | Jul 4, 2000 |
| Priority date | — |
| Expiry date | May 16, 2017 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA61K48/00
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
Composition comprising DNA associated with a polycation moiety wherein the polycation moiety is itself coupled to an integrin receptor binding moiety is disclosed. Preferably, the integrin receptor binding moiety is a peptide, and the compositions can be used to deliver DNA to a cell where it will be expressed, for example, to treat a condition by gene therapy. In a preferred embodiment, the integrin receptor binding moiety comprises a peptide, in particular a cyclic peptide, comprising the sequence RGD. In a particularly preferred embodiment, the peptide comprises the sequence GGCRGDMFGC. Cyclic configuration in this sequence is imposed by virtue of the presence of two cysteine residues which can form a disulphide bond.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.