Method of refolding human activin A
US6084076A · kind A · utility
12Cited by
4References
12Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Feb 8, 1999 |
| Grant date | Jul 4, 2000 |
| Priority date | — |
| Expiry date | Feb 8, 2019 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K14/575
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for industrially producing human activin A which comprises refolding a modified human activin A produced by a microorganism into natural-form human activin A having a biological activity. This method comprises the steps of: (a) solubilizing the modified human activin A with a modifier and then protecting the thiol groups with glutathione and/or sodium sulfite; and (b) dialyzing the protected modified human activin A with a refolding buffer, containing taurodexoycholic acid or its salts and then adding a thiol compound thereto conduct a disulfide bond interchange reaction.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.