PCR detection of Borrelia burgdorferi
US6087097A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | May 3, 1996 |
| Grant date | Jul 11, 2000 |
| Priority date | — |
| Expiry date | May 3, 2016 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY02A50/30
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for detecting in a biological sample the presence of Lyme-disease causing spirochetes using the polymerase chain reaction (PCR). This method includes the steps of: isolating DNA from the biological sample; amplifying the isolated DNA under hybridizing conditions with a primer pair that targets portions of extrachromosomal linear plasmid gene encoding outer surface protein A (OspA) or outer surface protein B (OspB) of the Lyme-disease causing spirochetes, probing the amplified DNA under hybridizing conditions with a labeled gene probe; and detecting the labeled gene probe that hybridized to the amplified DNA of the Lyme-disease causing spirochetes.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.