Optical characterization of nucleic acids and oligonucleotides

US6087101A · kind A · utility

Inventors

• Karl Otto Gruelich · Heidelberg, DE
• Claus Seidel · Göttingen, DE
• Juergen Wolfrum · Rosdorf, DE
• Manfred Auer · Zoznegg, DE
• Matthias Gautel · Karlsruhe, DE
• Roger S. Goody · Dortmund, DE
• Siegfried Labeit · Neckargemünd, DE

Key dates

• Filing date: Dec 15, 1997
• Grant date: Jul 11, 2000
• Expiry date: Dec 15, 2017

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q2523/319
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A method for the enzymatic sequencing of DNA is disclosed which uses dideoxythio-nucleotides or dideoxyamino-nucleotides as terminators that are labeled with one or two fluorescent dyes coupled to the terminators either before or after polymerization. The dye labeled DNA fragments are separated in a single lane of a separation system and then the terminal bases of each of the fragments are identified by measuring the fluorescent lifetime of the dye attached to the terminators.