Recombinant expression of proteins from secretory cell lines
US6087129A · kind A · utility
44Cited by
7References
26Claims
0Family size
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Key dates
| Filing date | Jan 19, 1996 |
| Grant date | Jul 11, 2000 |
| Priority date | — |
| Expiry date | Jan 19, 2016 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2840/203
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
The present invention a provides methods for production of heterologous polypeptides using a variety recombinantly engineered secretory cell lines. The common feature of these cell lines is the absence of expression of at least one endogenous polypeptide. The host cell machinery normally used to produce the endogenous polypeptide is then usurped for the purpose of making the heterologous polypeptide. Also described are methods engineering cells for high level expression, methods of large scale protein production, and methods for treatment of disease in vivo using viral delivery systems and recombinant cell lines.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.