Patent · US Expired

Cleavage of nucleic acids

US6090543A · kind A · utility

255Cited by
20References
27Claims
0Family size

Assignee

Inventors

Key dates

Filing dateDec 2, 1996
Grant dateJul 18, 2000
Priority date
Expiry dateDec 2, 2016

Classification

  • Technology area (CPC Y)Emerging Cross-Sectional Technologies
  • CPC primaryY10S435/822
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.