Automated DNA sequencing comparing predicted and actual measurements
US6090550A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Aug 28, 1997 |
| Grant date | Jul 18, 2000 |
| Priority date | — |
| Expiry date | Aug 28, 2017 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/143333
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method of automatically sequencing DNA comprises repeatedly determining the next base in the sequence as a function not only of a physical measurement made at that position, but also as a function of previously-determined near-by bases in the same sequence. Typically, a computer algorithm is used which predicts the value of the expected measurement at a given position based upon a knowledge of the bases in previous and/or subsequent positions. The predicted measurement is then compared with the actual measurement, and the base chosen at that position is the base which minimizes the accumulated error measure for the entire sequence. The preferred algorithm, which may be parallel or sequential, preferably includes physical modelling of the replication effect and of the fluorescence effect.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.