Use of uracil-DNA glycosylase in genetic analysis
US6090553A · kind A · utility
45Cited by
6References
27Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | Oct 29, 1997 |
| Grant date | Jul 18, 2000 |
| Priority date | — |
| Expiry date | Oct 29, 2017 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6858
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to a process for detecting the presence of at least one specific nucleic acid sequence in a sample containing a nucleic acid or a mixture of nucleic acids by amplifying the nucleic acid using polymerase chain reaction, cleaving the amplified products with uracil DNA glycosylase to obtain short DNA segments and detecting the DNA fragments by using reverse blot hybridization.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.