Patent · US Expired

Reducing nontemplated 3' nucleotide addition to polynucleotide transcripts

US6090590A · kind A · utility

12Cited by

0References

17Claims

0Family size

Assignees

The Regents of the University of California · US
Advanced Research and Technology · US

Inventor

C. Cheng Kao · Bloomington, US

Key dates

Filing date	Aug 10, 1999
Grant date	Jul 18, 2000
Priority date	—
Expiry date	Aug 10, 2019

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12Q1/6853
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

Non-template 3' nucleotide addition to a transcript is reduced by transcribing a transcript from a template comprising an ultimate and/or penultimate 5' ribose having a C'2 substituent such as methoxy, which reduces non-template 3' nucleotide addition to the transcript. The methods are shown to be applicable to a wide variety of polymerases, including Taq, T7 RNA polymerase, etc.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.