Enhanced expression of proteolytic enzymes in koji mold
US6090607A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | May 17, 1999 |
| Grant date | Jul 18, 2000 |
| Priority date | — |
| Expiry date | May 17, 2019 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K14/38
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention has for an object a koji mold which expresses at least 2 times more endo- and exo-peptidases than the wild type strain of Aspergillus oryzae CNCM I-1882, and especially at least 30 mU of endopeptidase activity, at least 30 mU of leucine-amino-peptidase and at least 10 mU of prolyldipeptidyl-peptidase activity per ml of supernatant when grown in a minimal medium containing 0.2% soy bean proteins. The invention also provides a DNA-binding protein of Aspergillus oryzae (AREA) having at least the amino-acid sequence from amino-acid 1 to amino-acid 731 of SEQ ID NO:2 or functional derivatives thereof. The invention also provides a DNA molecule that comprises an areA gene encoding the DNA-binding protein according to the invention. In a fourth aspect, the invention provides a method for over-producing proteolytic enzymes, comprising cultivating a koji mold according to the invention in a suitable growth medium under conditions where the mold expresses enzymes, and optionally isolating the enzymes in the form of a concentrate. In another aspect, the invention provides the a method for hydrolyzing protein-containing materials using the koji mold of the invention. In a…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.