Patent · US Expired

Octopine T-DNA structural genes

US6090627A · kind A · utility

25Cited by
3References
37Claims
0Family size

Assignee

Inventors

Key dates

Filing dateJun 2, 1995
Grant dateJul 18, 2000
Priority date
Expiry dateJun 2, 2015

Classification

  • Technology area (CPC Y)Emerging Cross-Sectional Technologies
  • CPC primaryY02A40/146
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

The sequence of the T-DNA of the octopine-type Ti plasmid found in Agrobacterium tumefaciens ATCC 15955 is disclosed. Fourteen open reading frames bounded by eukaryotic promoters, ribosome binding sites, and polyadenylation sites were found. The use of promoters and polyadenylation sites from pTi15955 to control expression of foreign structural genes is taught, using as examples the structural genes for the Phaseolus vulgaris storage protein phaseolin, P. vulgaris lectin, thaumatin, and Bacillus thuringiensis crystal protein. Vectors useful for manipulation of sequences of the structural genes and T-DNA are also provided.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.