Process for altering the host range or increasing the toxicity of Bacillus thuringiensis lepidoteran toxins, and recombinant DNA sequences therefor
US6090931A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | May 13, 1997 |
| Grant date | Jul 18, 2000 |
| Priority date | — |
| Expiry date | May 13, 2017 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12R2001/075
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention concerns an in vitro process for altering the insect host range (spectrum) or increasing the toxicity of lepidopteran active B.t. crystal protein toxins. The process comprises recombining in vitro the variable region(s) (non-homologous) of two or more genes encoding lepidopteran active B.t. crystal protein toxins. Specifically exemplified is the recombining of the variable regions of two genes obtained from well-known strains of Bacillus thuringiensis var. kurstaki. The resulting products are chimeric toxins which are shown to have an expanded and/or amplified lepidopteran insect host range as compared to the parent toxins.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.