Method for directing evolution of a virus
US6096548A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Feb 3, 1997 |
| Grant date | Aug 1, 2000 |
| Priority date | — |
| Expiry date | Feb 3, 2017 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/64
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention provides a number of strategies for transferring and/or evolving gene(s) associated with cellular DNA uptake so that they confer or enhance DNA-uptake capacity of a recipient cell. Evolution is achieved by recursive cycles of recombination and screening/selection. One such strategy entails evolving genes that confer competence in one species to confer either greater competence in that species, or comparable or greater competence in a second species. Another strategy entails evolving genes for use as components of cloning vector to confer enhanced uptake of the vector. Other strategies entail evolving viral receptors, viruses, and genes that mediate conjugal transfer.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.