Process for reverse transcriptase activity measurement using fluorescence polarization
US6100039A · kind A · utility
5Cited by
0References
19Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jan 28, 1999 |
| Grant date | Aug 8, 2000 |
| Priority date | — |
| Expiry date | Jan 28, 2019 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/68
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Described is a process for detecting reverse transcriptase activity and, thereby, reverse transcriptase inhibitors using fluorescence polarization, comprising, mixing a DNA primer with an RNA template. Then forming an RNA/DNA duplex utilizing the reverse transcriptase and removing the RNA from the RNA/DNA duplex to form single-stranded DNA. Finally, adding a fluorescent-labeled oligonucleotide complementary to the single-stranded DNA for hybridizing to the single-stranded DNA; and, measuring the fluorescence polarization.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.