Method for treating biopolymers, microorganisms or materials by using more than one type of magnetic particles
US6100079A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Dec 21, 1998 |
| Grant date | Aug 8, 2000 |
| Priority date | — |
| Expiry date | Dec 21, 2018 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/82
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Method and apparatus are described for purifying and separating biopolymers from a homogenized biological milieu using a pipette tip. The biopolymers are complexed with first magnetic particles. A magnet is applied to the pipette tip to attract the biopolymer/magnetic particle complex to the inner surface of the pipette tip adjacent to the magnet for separation from the biological milieu. The magnet is removed and the magnetic particles dissociated and removed from the biopolymers. Second magnetic particles are bound to the biopolymers. The magnet is replaced against the exterior surface of the pipette tip, thereby attracting the bound biopolymers to the inner surface of the pipette tip adjacent to the magnet to allow separation from impurities. The biopolymers are then labeled for detection, which may include detecting the concentration and identity of the biopolymer.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.