Apparatus for electroporation
US6103084A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Jun 6, 1995 |
| Grant date | Aug 15, 2000 |
| Priority date | — |
| Expiry date | Jun 6, 2015 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12M35/02
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
An apparatus for electroporation stores a level of charge, specified by a user, on a capacitor, which is delivered to a cuvette through an optically isolated high voltage switch. The capacitor is charged through a charging system, including a current mode pulse width modulation control circuit, which monitors the current in the primary winding of a transformer and supplies a pulse width modulated signal, limiting the current on every pulse to a level set by the microcontroller, to the controlling transistor in order to generate the drive to the primary winding of the transformer. A controlled amount of energy is transferred through each pulse to the capacitor. The microcontroller monitors the voltage on the capacitor up to a threshold level to predict the number of pulses necessary to store the requested amount of charge on the capacitor. The microcontroller will then count the number of pulses until the number of pulses necessary to store the requested amount of charge on the capacitor has been reached. At this point the requested voltage is delivered to the cuvette through the optically isolated high voltage switch comprised of sensitive gate SCRs coupled together in series. The …
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.