Methods and reagents for typing HLA class I genes
US6103465A · kind A · utility
14Cited by
6References
41Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Oct 3, 1995 |
| Grant date | Aug 15, 2000 |
| Priority date | — |
| Expiry date | Oct 3, 2015 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6881
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for typing HLA class 1 genes. The method entails first contacting a sample DNA with first and second amplification primers, wherein the first and second primers are each at least partially located in an exonic region. Next, using the first and second primers, a target sequence is amplified by the PCR to form an amplicon of the target sequence. Finally, the amplicon is detected with a sequence-specific detection means, e.g., DNA sequencing. The invention also includes specific amplification primers, specific sequencing primers, and kits especially adapted for use with the above HLA typing method.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.