CDT assay
US6103478A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Feb 19, 1998 |
| Grant date | Aug 15, 2000 |
| Priority date | — |
| Expiry date | Feb 19, 2018 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/255
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The invention provides a method of assessment of carbohydrate-deficient transferrin in a transferrin containing body fluid, said method comprising the steps of: PA1 i) obtaining a transferrin containing liquid sample of or derived from a said fluid; PA1 ii) contacting said sample with a source of iron ions; PA1 iii) subsequently contacting said sample with an anionic ion exchange resin at a pH such as to cause carbohydrate-deficient transferrin to be retained by said resin; PA1 iv) subsequently contacting said resin with an eluant serving to release carbohydrate-deficient transferrin into the eluate from said resin; PA1 v) collecting a volume of said eluate substantially free from tetra- and penta-sialo transferrin; and PA1 vi) assessing the transferrin variant content in said volume of eluate. By including at least a proportion of the trisialotransferrin in the eluate, it is possible to use relatively simple assessment techniques such as turbidimetry in the assay.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.