Spodoptera frugiperda single cell suspension cell line in serum-free media, methods of producing and using
US6103526A · kind A · utility
32Cited by
0References
16Claims
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Key dates
| Filing date | Oct 8, 1998 |
| Grant date | Aug 15, 2000 |
| Priority date | — |
| Expiry date | Oct 8, 2018 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2740/16222
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Disclosed and claimed is a new insect cell line, Sf900+, ATCC CRL-12579. The insect cell line was established from Lepidoptera, Noctuidae, Spodoptera frugiperda Sf-9 (ATCC CRL-1771) through multiple rounds of limiting dilution and selection in a serum-free insect medium supplemented with added human insulin. The insect cell line is useful in BEVS or as an adjuvant and has many characteristics and advantages. Also disclosed and claimed are recombinant proteins from recombinant baculovirus expression in insect cells such as Sf900+ cells, for instance, HA, NA, EPO, CD4, CEA, and thrombospondin.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.