Methods and materials for determining relative abundance of microorganisms in mixed populations
US6107033A · kind A · utility
Assignees
Inventors
Key dates
| Filing date | Aug 13, 1998 |
| Grant date | Aug 22, 2000 |
| Priority date | — |
| Expiry date | Aug 13, 2018 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6888
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to a method for determining the relative abundance of individual species or lower phylogenetic subgroups of microorganisms in a mixed population of several microorganisms comprising the steps of: 1) providing a set of labeled in situ hybridization cluster oligonucleotide probes; 2) hybridization of said probes with a sample of the mixed population, and 3) quantitative analysis of the number of labeled microorganisms. Further it relates to a method for analyzing dynamics in relative abundance of individual microorganisms in a mixed population. Further it relates to a set of probes which are cluster specific and which are provided with at least one label for use in a method of the invention and to a kit of parts for determining the relative abundance of individual species of microorganisms in a mixed population of several microorganisms comprising such probes which together with suitable materials for pre-treating the sample and/or suitable materials for carrying out hybridization and/or suitable materials for analysis of the result of the hybridization.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.