Method for detecting microorganisms by separation and culture on a gelled system, gelled system and assay kit therefor, and use thereof in microbiology
US6107053A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Dec 7, 1999 |
| Grant date | Aug 22, 2000 |
| Priority date | — |
| Expiry date | Dec 7, 2019 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/975
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A centrifuge tube and a kit for detecting the presence or absence of microorganisms, particularly bacteria and yeasts, in a liquid sample of a biological material. The centrifuge tube has a cylinder portion; a frustoconical portion; a capillary at the bottom of the tube; and contains a gel system for detecting microorganisms. The gel system includes a development phase of a gel containing microorganism culture medium and a reagent capable of inducing a detectable variation in optical measurement in the presence of microorganisms and a first phase intimate mixture which includes water originating wholly or partly from the culture medium and water-absorbing polymer particles so that, in the first phase intimate mixture, the polymer particles have a particular concentration and size.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.