Modified primer extension reactions for polynucleotide sequence detection
US6107061A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Sep 18, 1999 |
| Grant date | Aug 22, 2000 |
| Priority date | — |
| Expiry date | Sep 18, 2019 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/81
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to assays for the detection of nucleotide bases at predetermined locations on polynucleotides of interest. The embodiments of the invention include techniques relating to methods in which a primer extension reaction is designed to only extend a single nucleotide base. The invention, in part, relates to the improvement of adding corresponding non-labeled chain terminators to a primer extension reaction so as to increase the accuracy and reliability of nucleotide base determinations made by the assay. The invention is particularly useful when applied to assay methods in which the primer extension reaction is detected by a fluorescent dyes conjugated to chain terminators incorporated in the extension reactions. A first embodiment of the invention relates to methods for identifying a nucleotide base at the predetermined location on a polynucleotide of interest. Other embodiments of the invention include compositions for identifying a nucleotide base at a predetermined location on a polynucleotide of interest. One embodiment of such a composition is a mixture comprising a polynucleotide for analysis, an extendable polynucleotide that can hybridize of a spec…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.