Non-optimal Kozaks sequences
US6107477A · kind A · utility
17Cited by
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20Claims
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Key dates
| Filing date | Oct 6, 1998 |
| Grant date | Aug 22, 2000 |
| Priority date | — |
| Expiry date | Oct 6, 2018 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6897
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A non-optimal translational start site (Kozak) polynuclcotide sequence as set forth in SEQ ID NO:2 is provided. An expression vector for expressing a protein of interest, utilizing the non-optimal translational start site is also provided. Host cells containing the expression vector utilizing the non-optimal translational start site are also provided. A selectable marker encoded by a nucleic acid sequences utilizing the non-optimal translational start site is further provided.
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