Gene synthesis method

US6110668A · kind A · utility

Assignee

• Max-Planck-Gesellschaft zur Föderung der Wissenschaften e.V. · US

Inventors

• Nicolai Strizhov · Neunburg vorm Wald, DE
• Csaba Koncz · Neunburg vorm Wald, DE
• Jeff Schell · Cologne, DE

Key dates

• Filing date: Dec 20, 1996
• Grant date: Aug 29, 2000
• Expiry date: Dec 20, 2016

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y02A40/146
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A method of gene synthesis is disclosed. The gene synthesis method permits the codons of [m] natural gene to be changed to allow preferential transcription and translation of the synthetic gene in transgenic organisms. The method utilizes a combination of enzymatic and chemical synthesis of DNA and significantly reduces the cost, time and number of steps required for construction of synthetic genes.