Nucleotide sequences, probes and a process for the in vitro diagnosis of chromosomal anomalies correlated with CMT1A disease
US6110670A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Mar 4, 1997 |
| Grant date | Aug 29, 2000 |
| Priority date | — |
| Expiry date | Mar 4, 2017 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2600/172
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention relates to a process for the in-vitro diagnosis of chromosomal anomalies liable to be correlated with CMT1a disease. The invention also relates to probes intended in this in-vitro diagnosis process and to kits containing said probes. The probes of the invention can contain a sequence constituted of from about 15 successive nucleotides of a Not1 fragment, with said Not1 fragment having 1.2.times.10.sup.6 base pairs and being obtained after digesting human DNA of patients with Not1, separating the fragments resulting from digestion by pulsed field gel electrophoresis and hybridizing the resulting fragments with any of the probes VAW409, EW401 or VAW412 or their derivatives, to about the total number of the successive nucleotides of the Not1 fragment. The probes enable the detection of the duplication of a part of chromosome 17p.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.